International Journal of Environmental Science and Technology

Effect of trace metals on halophilic and mixed cultures in anaerobic treatment

1K. Krongthamchat, 2*R. Riffat and 3S. Dararat

Trace metals are known to stimulate the activity of anaerobic methanogenic bacteria involved in the degradation of organic matter. The objective of this research was to investigate the effect of three trace metals, iron, nickel and cobalt on the activity of halophilic methanogens, digester sludge and a mixed culture of halophilic bacteria and digester sludge, at various salt concentrations. Iron slightly stimulated both initial and maximum specific methanogenic activity (SMA) of halophilic methanogens at all salt concentrations. Iron addition was also able to increase both initial and maximum SMA of the mixed culture. Iron could not stimulate either initial or maximum SMA of digester sludge at salt concentrations of more than 10 g/L. Adding cobalt showed no benefit on the initial and maximum SMA of halophilic methanogens. A slight increase in the initial SMA was observed in mixed culture when cobalt was injected to the system containing sodium concentrations of 10-30 g/L. At salt concentrations above 40 g/L, cobalt could stimulate the maximum SMA of mixed culture. No benefits were found when cobalt was added to the digester sludge at sodium concentrations higher than 20 g/L. Nickel was observed to increase the initial SMA of digester sludge at all salt concentrations, and the maximum SMA of mixed culture at salt concentrations of 30-50 g/L.tions of 30-50 g/L.

Evaluation of benzene exposure in adults and urinary s-phenylmercapturic acid in children living in Adelaide, South Australia

1*A. R. Bahrami and 2J. W. Edwards

Benzene Exposure was evaluated in adults and children living in Adelaide, South Australia by measuring benzene and urinary s-phenylmercapturic acid (SPMA). To determine of benzene exposure in each subject the personal passive samplers was used and samples were analyzed by gas chromatography system equipped to flame ionization detector. The level of SPMA was determined by competitive enzymelinked immunosorbent assay (ELISA) in children. The mean concentration of benzene in Summer and Winter were 1.62±1.43 and 1.36±0.87 ppb respectively. There was a significant difference between exposure to benzene for subjects with less and more than 6 hours activity over days of week (p<0.05). The mean urinary concentrations levels of SPMA adjusted to creatinene for children that living less and more than 200 meters distance from main road were 1.56 and 4.67 mol/mol creatinene, respectively and the significant difference was seen in two groups (p<0.005). Data shows, that SPMA can be utilized as a biomarker for exposure to benzene in children. Exposure to benzene is more for children that living near to main road compare to other children. Adults have more activity in out side of home has more exposure to benzene than other people.

Unplanned urbanization: Assessment through calculation of environmental degradation index

1*Md. J. B. Alam, 2M. J. B. Alam, 2M. H. Rahman, 1S. K. Khan and 1G. M. Munna

Urbanization is an inevitable phenomena for the country like Bangladesh. The push and pull factors help to migrate people from rural area to urban area. As a result haphazard and unplanned urbanization create environmental degradation. Survey data reveals that inadequate solid waste disposal services, lack of adequate public water supply, traffic congestion, water logging, air pollution, noise pollution, hill cutting are the main problems in the city area. Concentration of dust as well as SOx, NOx exceeded the allowable limit at the selected points. Groundwater level of Sylhet city has a considerable lowering over the last few decades. It has lowered from 3380 mm below ground in 1982 to 7880 mm below ground. Sylhet is located in highly seismic risk zone but most of the buildings and other structures in this area are constructed without considering earthquake risk. Environmental degradation index was calculated based on economical condition of the country.

Lead remediation of contaminated water using Moringa Stenopetala and Moringa oleifera seed powder

L. M. Mataka, *E. M. T. Henry, W. R. L. Masamba and S. M. Sajidu

The increasing influx of heavy metals into water bodies from industrial, agricultural, and domestic activities is of global concern because of their well documented negative effects on human and ecosystem health. A recent study of streams in Blantyre and Zomba, Malawi revealed lead levels of up 0.118 mg/L, exceeding the World Health Organisation acceptable level of 0.01 mg/L. Our ongoing study on low cost effective heavy metal remediation techniques in developing countries has already demonstrated that Moringa oleifera, the well known source of natural water clarifiers, is effective in heavy metal detoxification of water. This paper presents the first reported use of a related species, the African moringa, Moringa stenopetala for lead detoxification and preliminary investigation of the interaction of the metal with the polyelectrolytes of M. oleifera and stenopetala. The potential of M. stenopetala for lead removal was tested by means of jar tests. With an initial lead concentration of 7 ppm, M. stenopetala seed powder, at doses of 0.50, 1.00, 1.50, 2.00 and 2.50 g/100mL, reduced the concentration of lead by 20.00 ± 0.00, 46.19 ± 2.06, 71.19 ± 2.06 and 89.43 ± 0.60 and 96.23 ± 0.12 % respectively. M. stenopetala was more effective than M. oleifera in removing lead from water (p=0.001 at 95% confidence level). For oleifera, lead levels decreased exponentially during the first 5 h. of the reaction and then equilibrium was established; for stenopetala, a linear decrease was observed. The pH of the mixture rose from 2.30 to a maximum of 2.53 and 2.57 and then fell to an equilibrium value of 2.30 and 2.29 for oleifera and stenopetala respectively. Lead removal was also affected by pH, ionic strength, and water hardness. Our results show that M. stenopetala has potential in lead remediation of contaminated waters. Further studies are being carried out on remediation of other metals and the mechanism of the metal moringa interaction.

Utilization and flow of energy in tropical ecosystem: A test case for two Ox-bow lakes

1*S. K. Das and 2D. Chakrabarty

To estimate the rate of organic production, two solar powered tropical natural aquatic systems (Ox-bow lakes) have been studied for 24 months. The study of utilization of energy in those two tropical lakes has revealed that herbivores were more efficient in energy utilization than autotrophs. Silver carp and grass carp were found to be most efficient in conversion of energy in the grazing and detritus food chain of these lakes. The efficiency of the system to fish production was found to be 0.05 % of the total available solar radiation. Various forms of life are all accompanied by energy changes, even though no energy is created or destroyed in the studied system.

Pollution of south of Tehran ground waters with heavy metals

1*R. Salmasi and 2 A. Tavassoli

The reuse of nutrients and organic matter in wastewater sludge via on agricultural lands application is a desirable goal. However, trace or heavy metals present in sludge pose the risk of human or phytotoxicity from land application. The aim of this research is possibility of ground water pollution of south of Tehran because of ten years irrigation with Ni, Cd and Pb borne waste water. For this purpose, 6 soil samples from southern parts of Tehran city and 2 ones from Zanjan city without lime and organic matter were selected. The soils differed in their texture from sandy to clayey. Each soil sample in duplicate and uniformly packed into PVC columns. Soil samples were irrigated with Cd, Pb and Ni-added wastewater. After irrigating, the columns were cut and the soils separated from sectioned pieces and their heavy metal concentrations (Pb, Cd and Ni) were measured by atomic absorption spectrophotometer by use of HNO₃ 4N solution. Because of high sorption capacity of these elements by soils, these metals were accumulated in surface layer of the soils. Movement in the soils without lime and organic matter were as low as other samples. Ni has had the most accumulation or the least vertical movement, and Pb the opposite ones.

The removal of color and COD from wastewater containing water base color by coagulation process

*H. Asilian, Sh. Moradian fard, A. Rezaei, S. B. Mortazavi and A. Khavanin

In this study acrylic water base color was removed from synthetic wastewater using coagulation process. Experiments were conducted on the sample containing 100 mg/L and 400 mg/L of acrylic water base color. Destruction of color by means of coagulation/flocculation techniques using ferrous sulfate, alum, lime and polyelectrolyte (cationic, anionic and non ionic).The study was performed in a systematic approach searching optimum values of alum and FeSO₄ concentration, pH and temperature. All the experiments were run in a laboratory scale .The obtained results show that treatment with alum and ferrous sulfate alone proved to be very effective in removing the color (> 99 %) and part of COD (60-70 %) from aqueous solution. Lime alone did not significant change on COD and color removal.

Microbiologically influenced corrosion in dairy effluent

*B. Ramesh Babu, S. Maruthamuthu, A. Rajasekar, N. Muthukumar and N. Palaniswamy

In the dairy industry mild steel is used as the construction material for the effluent treatment plants, pipelines, reinforcement in concrete structures etc. The steel exposed to the dairy effluent faces corrosion due to the microbes. In the present study the role of microbes in dairy effluent on the corrosion of mild steel has been investigated. Pseudomonas sp., Streptococcus sp., Micrococcus sp., Bacillus sp., Neisseria sp. and Lactobacillus sp. were identified in dairy effluent. Corrosion rate has been estimated by weight loss measurements and polarization technique. The Fourier transform infrared spectroscopy (FTIR) and X-ray diffractometer (XRD) studies were found helpful in investigating the chemical pathway leading to the formation of corrosion products on the mild steel during fermentation. Initiation of pitting corrosion was noticed on steel specimens by scanning electron microscope (SEM). A mechanism has been proposed for microbiologically influenced corrosion in dairy effluent.

Preliminary assessment of heavy metals levels of soils of an oil field in the Niger Delta, Nigeria

1*C. M. A. Iwegbue, 2F. E. Egobueze and 3K. Opuene

This communication presents the results of preliminary investigation of the characteristic levels of heavy metals in surface soils of an oilfield in the Niger Delta. The results indicate higher concentration of the following metals: Cd, Pb, Cu; Ni, Zn, Cr, Mn and Hg in soils around the gas plant than the pipeline areas. There is a significant temporal and spatial variation in the concentrations of the heavy metals. Samples collected during the wet season showed lower concentrations of heavy metals. The distribution pattern of heavy metals follows the following order Fe> Mn> Zn> V> Cr> Pb> Cu> Ni> Cd> Hg> As. The soils around the oilfield could be considered unpolluted since the concentrations of the metals fit into background levels and concentrations found in natural and agricultural soils. Since metal build up is a gradual process, farmland, fishing ponds and water bodies closer to these facilities will be at risk of heavy metal pollution over time.

Effect of aluminum and chromium on the growth and germination of mesquite (Prosopis juliflora swartz.) DC.

1Sh. N. Jamal, 1M. Z. Iqbal and 2*M. Athar

Prosopis seeds were grown under controlled environment in solution of aluminum and chromium at different concentration alone as well as combined together. The effect of these metals was studied on seed germination, root length, shoot length, seedling length and dry biomass. Aluminum and chromium alone, and combined together showed no effects on germination and dry biomass. Chromium alone was found toxic to root, shoot and seedling length. However, application of different concentrations of aluminum increased the root, shoot and seedling growth. It may be concluded that aluminum is not as toxic as chromium, and their combined treatment showed the intermediate effect by ameliorating the impact of one another.

Cadmium absorption and accumulation in different parts of kidney beans, radishes and pumpkins

A. A. Azimi, *T. Navab Daneshmand and A. Pardakhti

This study is done to measure the absorption and distribution of cadmium in different parts of kidney beans, radishes and pumpkins. Three parts of a field was chosen. In one part 65 ppm of cadmium nitrate was added to water and in the other part 130 ppm, the last part was irrigated with normal water. Samples were digested by EPA 3050 method. Cadmium concentration was measured by Unicam 919 absorption unit. Beans accumulate cadmium mostly in root (70 ppm) and a little amount is mobilized through upper parts (12-16ppm), but kidneys did not accumulate a significant amount. In radishes the roots did not accumulate a significant amount of cadmium but stems had 4 ppm and leaves had 25 ppm. Cadmium concentration in soil does not affect its concentration in different parts of pumpkins and beside the stems and leaves (4 ppm) the other parts' concentrations were insignificant. In regard to the results of this study the cadmium concentrations in edible parts of the samples (kidney beans, radish roots and pumpkin fruit) were less than the U.S. EPA standards for agriculture and human beings.

Isolation and characterization of some polycyclic aromatic hydrocarbon degrading bacteria from Nsukka soils in Nigeria

*C. I. Nnamchi, J. A. N. Obeta and L. I. Ezeogu

Twenty-four bacteria capable of utilizing naphthalene, as their sole source of carbon and energy for growth were isolated from three different sites in Nsukka, Nigeria. By standard bacteriological methods, these bacteria were characterized taxonomically as belonging to the genus Pseudomonas, Burkholderia or Actinomycetes. Two of the isolates, which showed the highest growth during screening as demonstrated by an increase in their optical densities (OD₆₀₀) and identified as Pseudomonas aeruginosa and Burkholderia cepacia respectively, were also able to grow in anthracene and carbazole, but not very much so in 2,4-dichlorophenol and D-camphor. The isolates showed a concentration-dependent growth in all the compounds they grew in. There were visible changes in the colour of the growth medium of the isolates during their incubation, suggesting the production of different metabolites. There were also changes in their medium pH during growth. These studies demonstrate the possession by the bacterial species of novel degradative systems.

Genotypic and environmental interaction in advanced lines of rice under salt-affected soils of Punjab

*Y. Ali, G. Sarwar, Z. Aslam and F. Hussain

Sixteen genotypes developed through hybridization and mutation breeding techniques were evaluated during the year 2003-04 on nine locations covering most of the area of Punjab province including Faisalabad, Toba Tek Singh and Jhang. NIAB-IRRI-9, Jhona-379 salt tolerant genotypes and super Basmati, a salt sensitive variety were included as standard checks. The tested material (six genotypes) was generated by crossing fine varieties of rice viz. Basmati-370 and Basmati-385 with three coarse varieties of rice viz. NIAB-IRRI-9, Jhona-349 and NIAB-Rice-1. The mutants (seven mutants) were derived by gamma irradiation of rice varieties, Basmati-370 and Basmati-385. The nine sets of experiments were conducted in the naturally high saline sodic soils having EC range of 9.2-18.13 d/Sm pH=8.65-9.50, and SAR=20.7-37.15. The seed yield data collected at maturity and stability parameters were computed to know genotypic x environment interaction and varietals performance. The combined analysis of variance showed highly significant variance in case of varieties, locations and varieties x locations interaction. Varieties and varieties x Environment (Lin) also showed highly significant differences. It means that genotypes exhibited different behavior in different locations / environments which is due to their different genetic make up. The behavior may be cross over (in which significant change in ranking occurs from one environment to another) or cross over nature (in which case the ranking of genotype remains constant across environments and the interaction is significant because of change in the magnitude of response) depending upon the ranking order of genotypes performance under different environment. In our study, only super Basmati behavior was of almost non cross over nature, while others had cross over type of interaction. Based on overall mean seed performance, mutant NIAB-Rice-4 and DM-38/88 produced significantly the highest grain yield (5.6 t/ha) followed by mutant line NIAB-Rice-5 (5.4 t/ha) and NIAB-rice-2 (5.3 t/ha). The lowest performing genotype was super Basmati (3.0 t/ha). The coarse variety Jhona-349 produced (4.8 t/ha) grain yield. Mutant line DM-38/88 showed significant regression co-efficient differences from unity while mutant line NIAB-Rice-4 showed non significant difference of regression co-efficient from unity. Mutant line, NIAB-Rice-4 also showed non significant difference of standard deviation to regression co-efficient from zero. All the three parameters of stability i.e. overall mean seed yield, regression co-efficient and standard deviation to regression, provided clear evidence that mutant line NIAB-Rice-4 produced maximum stable yield compared to super Basmati.

The role of urban forest in the protection of human environmental health in geographically-prone unpredictable hostile weather conditions

1*E. C. Anyanwu and 2I. Kanu